Dataset: Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)

Protein content was determined spectrophotometrically using the Bio-Rad Coomassie Blue assay in the microtiter plate protocol (BioRad Life Sciences Research, CA). At each sampling, 1 group of 8 larvae from each tub within each tank (i.e., 2 replicates tank-1) was frozen in liquid nitrogen and stored. Proteins were solubilized in 0.1 M NaOH, aided by sonication (10% amplitude for 15 s using a Branson Digital Sonifier S-250D, USA) and warming at 50 °C for 5 h. The extract was neutralized with 1 M HCl and processed in triplicate with the addition of the dye reagent. Following 30 min incubation, absorbances were measured at 595 nm using a plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA), and converted to protein using a calibration prepared from bovine serum albumin. Protein content was expressed as microgram larva-1.

The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa
The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C

Data also available from PANGAEA: doi:10.1594/PANGAEA.823582