Dataset: NCBI links to BioProjects of total RNA isolated from Trematomus bernacchii gill tissues acclimated to elevated temperature and pCO2, July 2015

Specimens of Trematomus bernacchii, Pagothenia borchgrevinki, and Trematomus newnesi were collected by hook and line near the Delbridge Islands in McMurdo Sound (77°40′S 166°25′E ) from October through December, 2011 and September through December, 2012. Once collected, fish were transported back to McMurdo Station in aerated coolers where they were acclimated for one week in a flow-through aquarium (2400-L) in ambient seawater (-1.5°C and ~430µatm CO2). After the initial acclimation period, fish were randomly placed into one of four flow-through experimental treatment tanks (1240-L each) in order to assess the response to increased temperature (4°C), increased pCO2 (1000µatm) or a combination of increased temperature and increased pCO2. The treatment tanks consisted of a tank held at ambient conditions (control treatment; -1°C and 430µatm), a low temperature + high pCO2 tank (-1°C and 1000µatm), a high temperature + low pCO2 tank (4°C and 430µatm), and a high temperature + high pCO2 tank (4°C and 1000µatm). Trematomus bernacchii, Pagothenia borchgrevinki were sampled after t= 7, 28, and 56 days of acclimation to the experimental conditions. Trematomus newnesi were sampled after t= 7, 28, and 42 days of acclimation. Four biological replicates from each treatment and time point were selected for cluster generation using the Illumina® TruSeq RNA Sample Prep v2 Hs Protocol and sequencing via an Illumina® HiSeq 2500 Rapid Run initialized for both paired-end 150bp reads and single-end 100bp reads.