Dataset: Microbial enzyme activities: peptidase activities in bulk seawater samples from the RV\Polarstern cruise ARKXXVII/3 in the Central Arctic Ocean and Laptev Sea, Aug-Sept. 2012

Final no updates expectedDOI: 10.26008/1912/bco-dmo.742780.1Version 1 (2018-07-24)Dataset Type:Cruise ResultsDataset Type:experimental

Principal Investigator: Carol Arnosti (University of North Carolina at Chapel Hill)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)


Abstract

This dataset includes peptidase activities measured in bulk (not filter-fractionated) seawater. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to mea...

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This dataset includes peptidase activities measured in bulk (not filter-fractionated) seawater. Links to archived CTD data are also provided. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid – leucine – and four oligopeptides – the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and glutamic acid-gylcine-arginine (EGR) – were used to measure exo- and endo-acting peptidase activities, respectively.


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Results

Balmonte, J. P., Teske, A., & Arnosti, C. (2018). Structure and function of high Arctic pelagic, particle‐associated and benthic bacterial communities. Environmental Microbiology, 20(8), 2941–2954. Portico. https://doi.org/10.1111/1462-2920.14304
Methods

Arnosti, C. (1996). A new method for measuring polysaccharide hydrolysis rates in marine environments. Organic Geochemistry, 25(1-2), 105–115. doi:10.1016/s0146-6380(96)00112-x
Methods

Arnosti, C. (2003). Fluorescent derivatization of polysaccharides and carbohydrate-containing biopolymers for measurement of enzyme activities in complex media. Journal of Chromatography B, 793(1), 181–191. doi:10.1016/s1570-0232(03)00375-1
Methods

Hoppe, HG. (1993). Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria, p. 423-431. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, FL 978-0873715645
Methods

Obayashi, Y., & Suzuki, S. (2005). Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases. Limnology and Oceanography, 50(2), 722–726. doi:10.4319/lo.2005.50.2.0722