Dataset: Calcification and carbonate chemistry from outdoor flume experiments with corals at locations in Moorea, Okinawa, and Hawaii from 2011 to 2013

See Comeau et al. (2014) for full methodology details.

Locations:  
UCB Gump Research Station, Moorea, French Polynesia
Sesoko Station, Tropical Biosphere Research Center, Okinawa, Japan
Hawaii Institute of Marine Biology (HIMB), Coconut Island, Oahu, Hawaii

Methodology:

At the three locations, pH was measured twice daily using a porta- ble pH meter (Orion 3-stars) fitted with a DG 115-SC pH probe (Mettler) calibrated every other day with Tris/HCl buffers (A. Dickson, San Diego, CA, USA). In Moorea and in Hawaii, pH also was measured spectrophotometrically once a week using m- cresol dye.T was calculated using a modified Gran function applied to pH values ranging from 3.5 to 3.0. Parameters of the carbonate system in seawater were calculated from salinity, temperature, AT and pHT using the R package seacarb.

To quantify net calcification, the difference between the initial and final buoyant weight after 14 days of incubation was converted to dry weight using an aragonite density of 2.93 g cm-3 for corals and Halimeda macroloba, and a calcite density of 2.71 g cm-3 for Porolithon onkodes. Net calcification was normalized to surface area, obtained using the foil technique for corals, and image analysis (IMAGEJ, US NIH) of aerial photographs for P. onkodes.

Species list:

Halimeda macroloba (macroalga). AphiaID 211524
Pocillopora damicornis (coral). AphiaID 206953
Porolithon onkodes (macroalga). AphiaID 495983
massive Porites (coral). AphiaID 206485