Dataset: Alexandrium cell counts from CTD bottle sampling from 2012 to 2019 in the Gulf of Maine

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.834443.1Version 1 (2021-05-04)Dataset Type:Cruise Results

Principal Investigator: Dennis J. McGillicuddy (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Bruce Keafer (Woods Hole Oceanographic Institution)

Data Manager: Olga Kosnyrev (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Program: Woods Hole Center for Oceans and Human Health (WHCOHH)

Project: WHCOHH - Physiological and behavioral plasticity in harmful algal bloom dynamics: variation across different habitats (WHCOHH Algal Bloom Dynamics)


Abstract

Alexandrium cell counts from CTD bottle sampling from 2012 to 2019 in the Gulf of Maine

Standard station CTD profiles measurements (down casts) with water sampling (up casts);

Calculations of the concentration (cells/Liter) of Alexandrium catenella, formerly A. fundyense, were completed using MS Excel. The spreadsheet used inputs of the original volume sieved at sea (usually 2 Liters), the volume re-suspended into formalin and then methanol (usually 14ml), and the volume of the plankton concentrate filtered for the molecular probe assay (usually 7ml,  or less volume if during a significant bloom), and the number of labeled cells observed on that filter.


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Methods

Anderson, D. M., Kulis, D. M., Keafer, B. A., Gribble, K. E., Marin, R., & Scholin, C. A. (2005). Identification and enumeration of Alexandrium spp. from the Gulf of Maine using molecular probes. Deep Sea Research Part II: Topical Studies in Oceanography, 52(19-21), 2467–2490. doi:10.1016/j.dsr2.2005.06.015