| Data File(s) | Type | Description | Action |
|---|---|---|---|
CompDataProcessed.csv (11.50 KB) | Comma Separated Values (.csv) | CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript. | |
CompDataProcessed.outlierremoved.csv (23.93 KB) | Comma Separated Values (.csv) | CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript. | |
GRR.csv (2.82 KB) | Comma Separated Values (.csv) | GRR.csv These represent the data summarized in manuscript Figure 2. Strain: Cyanobacterial strain. Dataset: Either this study (Morris) or our previous meta-analysis (Dutkiewicz). Genus: Either Prochlorococcus, Synechococcus, or "Diazotroph". Flag: Either Prochlorococcus (Pro) or not (NotPro). CO2-r, CO2+r: malthusian growth rate at 400ppm or 800ppm pCO2 respectively. Only available for experiments from this study; previous reports summarized in Dutkiewicz et al 2015 only included exponential growth rates. CO2-x, CO2+x: as above, but exponential growth rates. rgrr, xgrr: "growth rate response" for malthusian and exponential growth rates, calculated as described in the manuscript. | |
GrowthRates.csv (28.03 KB) | Comma Separated Values (.csv) | GrowthRates.csv: These represent the data summarized in manuscript Figure 1. Strain: Cyanobacterial strain. Genus: either Prochlorococcus or Synechococcus. CO2: Either 400 ppm (CO2-) or 800 ppm (CO2+) pCO2. Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text. | |
KnightDataAnalysis.txt (54.49 KB) | Octet Stream | To re-run the analyses, place all the files in one directory, set that directory to the working directory in R, and copy-and-paste the entire contents of the file KnightDataAnalysis.txt into the R window and hit "enter". You may need to install the plyr, lme4, and emmeans packages beforehand. | |
ReadMe.txt (4.25 KB) | Octet Stream | ReadMe file describing all the other files and how to use them. | |
Syn-Pro_Co-cultures.zip (42.19 KB) | ZIP Archive (ZIP) | .zip package containing all the files needed to re-run the analyses performed in the paper "Co-culture with Synechococcus facilitates Prochlorococcus growth under ocean acidification conditions". | |
Together.csv (24.26 KB) | Comma Separated Values (.csv) | Together.csv This is the data summarized in manuscript Figure 5. It includes all the cultures from CompDataProcessed.outlierremoved.csv as well as comparison cultures with only one of the two cyanobacterial strains. Strain: Either Prochlorococcus or Synechococcus. Together: Row either corresponds to data collected from unialgal cultures (No) or co-cultures of both strains (Yes). Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text. |
Files
Filename: CompDataProcessed.csv (11.50 KB)
Type: Comma Separated Values (.csv)
Description: CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript.
Type: Comma Separated Values (.csv)
Description: CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript.
Filename: CompDataProcessed.outlierremoved.csv (23.93 KB)
Type: Comma Separated Values (.csv)
Description: CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript.
Type: Comma Separated Values (.csv)
Description: CompDataProcessed.csv and CompDataProcess.outlierremoved.csv These are the co-culture competition experiments summarized in manuscript Figures 3 and 4. Experiment: Two experimental blocks were performed, one by author Knight (Maggie) and one by author Morris (Jeff). Culture: Each competition paired one of 6 Prochlorococcus clones, designated as LTPE 26-31, with one of 6 Synechococcus clones, designated LTPE 45-50. CO2: Either 400 ppm (0) or 800 ppm (1) pCO2. Replicate: In some cases multiple replicates of a particular clone pairing were performed, this column indicates which replicate a given row corresponds to. Transfer: Each culture was transferred into fresh media at least 2 times, giving at least 3 separate cultures in sequence. This column indicates which sequential transfer each row represents. Time: Time (days) between inoculation and transfer. InitPro, InitSyn, FinalPro, FinalSyn: densities in cells/mL of Prochlorococcus or Synechococcus at the beginning and end of the transfer. ProGR, SynGR: malthusian growth rate of Prochlorococcus and Synechococcus respectively, calculated as described in the manuscript. InitProFreq: Initial frequency (0-1) of Prochlorococcus. Wpro: Fitness calculated as the ratio of the Prochlorococcus malthusian growth rate vs. the Synechococcus malthusian growth rate. Dimensionless. Spro: Fitness calculated as the difference of the Prochlorococcus and Synechococcus malthusian growth rates. Units d^-1. InitDens, LogInitDens: Total cyanobacterial cell density at the beginning of the experiment. ProExGR, SynEXGR: as ProGR/SynGR, but using the exponential growth rate. WproEX, SproEx: as Wpro and Spro, but calculated using the exponential growth rates. LagPro, LagSyn: lag time in days, calculated as described in the manuscript.
Filename: GRR.csv (2.82 KB)
Type: Comma Separated Values (.csv)
Description: GRR.csv These represent the data summarized in manuscript Figure 2. Strain: Cyanobacterial strain. Dataset: Either this study (Morris) or our previous meta-analysis (Dutkiewicz). Genus: Either Prochlorococcus, Synechococcus, or "Diazotroph". Flag: Either Prochlorococcus (Pro) or not (NotPro). CO2-r, CO2+r: malthusian growth rate at 400ppm or 800ppm pCO2 respectively. Only available for experiments from this study; previous reports summarized in Dutkiewicz et al 2015 only included exponential growth rates. CO2-x, CO2+x: as above, but exponential growth rates. rgrr, xgrr: "growth rate response" for malthusian and exponential growth rates, calculated as described in the manuscript.
Type: Comma Separated Values (.csv)
Description: GRR.csv These represent the data summarized in manuscript Figure 2. Strain: Cyanobacterial strain. Dataset: Either this study (Morris) or our previous meta-analysis (Dutkiewicz). Genus: Either Prochlorococcus, Synechococcus, or "Diazotroph". Flag: Either Prochlorococcus (Pro) or not (NotPro). CO2-r, CO2+r: malthusian growth rate at 400ppm or 800ppm pCO2 respectively. Only available for experiments from this study; previous reports summarized in Dutkiewicz et al 2015 only included exponential growth rates. CO2-x, CO2+x: as above, but exponential growth rates. rgrr, xgrr: "growth rate response" for malthusian and exponential growth rates, calculated as described in the manuscript.
Filename: GrowthRates.csv (28.03 KB)
Type: Comma Separated Values (.csv)
Description: GrowthRates.csv: These represent the data summarized in manuscript Figure 1. Strain: Cyanobacterial strain. Genus: either Prochlorococcus or Synechococcus. CO2: Either 400 ppm (CO2-) or 800 ppm (CO2+) pCO2. Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text.
Type: Comma Separated Values (.csv)
Description: GrowthRates.csv: These represent the data summarized in manuscript Figure 1. Strain: Cyanobacterial strain. Genus: either Prochlorococcus or Synechococcus. CO2: Either 400 ppm (CO2-) or 800 ppm (CO2+) pCO2. Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text.
Filename: KnightDataAnalysis.txt (54.49 KB)
Type: Octet Stream
Description: To re-run the analyses, place all the files in one directory, set that directory to the working directory in R, and copy-and-paste the entire contents of the file KnightDataAnalysis.txt into the R window and hit "enter". You may need to install the plyr, lme4, and emmeans packages beforehand.
Type: Octet Stream
Description: To re-run the analyses, place all the files in one directory, set that directory to the working directory in R, and copy-and-paste the entire contents of the file KnightDataAnalysis.txt into the R window and hit "enter". You may need to install the plyr, lme4, and emmeans packages beforehand.
Filename: ReadMe.txt (4.25 KB)
Type: Octet Stream
Description: ReadMe file describing all the other files and how to use them.
Type: Octet Stream
Description: ReadMe file describing all the other files and how to use them.
Filename: Syn-Pro_Co-cultures.zip (42.19 KB)
Type: ZIP Archive (ZIP)
Description: .zip package containing all the files needed to re-run the analyses performed in the paper "Co-culture with Synechococcus facilitates Prochlorococcus growth under ocean acidification conditions".
Type: ZIP Archive (ZIP)
Description: .zip package containing all the files needed to re-run the analyses performed in the paper "Co-culture with Synechococcus facilitates Prochlorococcus growth under ocean acidification conditions".
Filename: Together.csv (24.26 KB)
Type: Comma Separated Values (.csv)
Description: Together.csv This is the data summarized in manuscript Figure 5. It includes all the cultures from CompDataProcessed.outlierremoved.csv as well as comparison cultures with only one of the two cyanobacterial strains. Strain: Either Prochlorococcus or Synechococcus. Together: Row either corresponds to data collected from unialgal cultures (No) or co-cultures of both strains (Yes). Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text.
Type: Comma Separated Values (.csv)
Description: Together.csv This is the data summarized in manuscript Figure 5. It includes all the cultures from CompDataProcessed.outlierremoved.csv as well as comparison cultures with only one of the two cyanobacterial strains. Strain: Either Prochlorococcus or Synechococcus. Together: Row either corresponds to data collected from unialgal cultures (No) or co-cultures of both strains (Yes). Time: The number of days between culture inoculation and transfer into fresh media. DensInit: Culture density at inoculation, in cells/mL. DensFinal: Culture density at transfer, in cells/mL. RealizedGR, ExponentialGR: malthusian and exponential growth rates in d^-1, calculated as described in the text. Lag: lag time in days, calculated as described in the text.
