Dataset: Volume effects on blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted from 2017-2020 (Biological Nitrogen Isotope Fractionation project)

Final no updates expectedDOI: 10.26008/1912/bco-dmo.865089.1Version 1 (2021-11-16)Dataset Type:experimental

Principal Investigator: Julie Granger (University of Connecticut)

Student, Contact: Mengyang Zhou (University of Connecticut)

Analyst: Bonnie X. Chang (University of Washington)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)


Project: CAREER: The biological nitrogen isotope systematics of ammonium consumption and production (Biological Nitrogen Isotope Fractionation)


Abstract

Volume-dependent N2O blanks originating from the bacterial concentrates, equilibration with atmospheric N2O, and NO3- contamination of the water into which the standards were diluted.

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Effects of sample volume on the size of bacterial blanks

Following sparging with N2 gas, bacterial concentrates (2 mL) in 20 mL vials were injected with incremental volumes of DIW or NO3--deplete surface Sargasso seawater. Solutions included air-equilibrated DIW (for ≥ 1 day) and helium-sparged DIW (for 30 minutes), as well as air-equilibrated vs. helium-sparged seawater. N2O yields were estimated from peak areas recovered by mass spectrometric analysis, calibrated with standard additions.


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Methods

Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., & Hilkert, A. (2002). Measurement of the Oxygen Isotopic Composition of Nitrate in Seawater and Freshwater Using the Denitrifier Method. Analytical Chemistry, 74(19), 4905–4912. doi:10.1021/ac020113w