©2020 Biological and Chemical Oceanography Data Management Office.Microsatellite genotypes and chloroplast 23S genotypes (based on length heteroplasmy in domain V of chloroplast large subunit (cp23S) ribosomal DNA sequences) of Breviolum sp. symbionts within Orbicella faveolata adults in the Florida Keys.
Sampling and Analytical Methodology
Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992).
Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009).
Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).
Locations in the Florida Keys (Table of reef abbreviations)
Reef
Abbreviation
Alligator
AR
Coral Garden
CG
Cheeca Rocks
CR
East Turtle
ET
Grecian Rocks
GR
Looe Key
LK
Sand Island
SI
Tennessee
TR
Coffroth, M. A., Miller, M. W., Sheets, D. (2023) Microsatellite and chloroplast 23S genotypes of Breviolum sp. symbionts within Orbicella faveolata adults from reefs in the Florida Keys from 2009-2011 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.882086.1 [access date]
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