Dataset: Chemotaxis of Vibrio alginolyticus to control/phage-infected Synechococcus exudates from 2020-2021 (VIC project)

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.885574.1Version 1 (2022-12-19)Dataset Type:model resultsDataset Type:experimentalDataset Type:model resultsDataset Type:experimental

Co-Principal Investigator: Sheri Floge (Wake Forest University)

Co-Principal Investigator: Jeffrey Guasto (Tufts University)

Scientist, Contact: Richard J. Henshaw (ETH-Zurich)

BCO-DMO Data Manager: Sawyer Newman (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral induced chemotaxis mediating cross-trophic microbial interactions and carbon flux (VIC)

Abstract

This data set summarises the chemotactic response of a model marine bacteria (Vibrio alginolyticus YM4 wild-type) to filtered exudates of the cyanobacteria Synechococcus sp WH8102. Two filtrate sets were collected, each spanning 6 time points (named T1 -> T6), with the initial assays split into 4 biological replicates (named A,B,C,D). The two treatments were: 1) A control treatment (named "Control", or shortened to "C") 2) A phage-infected treatment (named "Phage", or shortened to "P"), wher...

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The data in this data set is organized as follows:

Accumulation Curves: The final processed data for each time point (T1-6), each replicate (A-D) with the average and SEM values tabled. 

Filtrates_T#R: Where # is the time point number (1-6) and R the replicate (A-D). Each folder contains:

1) Three Excel sheets corresponding to the raw cell counts for each biological replicate (each with 3 repeats)

2) A supplemental folder containing a cell array with the full bacteria positions for each time point for each replicate (.mat format)

The chemotaxis data was collected by Dr. Richard J. Henshaw of Tufts University (Boston, MA, USA) from 3rd December 2020 to 8th October 2021. Queries about this dataset should be directed to rhenshaw@ethz.ch.

Related Datasets

IsRelatedTo
Dataset: Chemotaxis of V. alginolyticus towards Synechococcus Cells
Floge, S., Guasto, J., Henshaw, R. J. (2023) Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells (VIC project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-10-17 http://lod.bco-dmo.org/id/dataset/913619

Related Publications

Methods
Dehkharghani, A., Waisbord, N., Dunkel, J., & Guasto, J. S. (2019). Bacterial scattering in microfluidic crystal flows reveals giant active Taylor–Aris dispersion. Proceedings of the National Academy of Sciences, 116(23), 11119–11124. https://doi.org/10.1073/pnas.1819613116
Methods
Howard-Varona, C., Roux, S., Bowen, B. P., Silva, L. P., Lau, R., Schwenck, S. M., Schwartz, S., Woyke, T., Northen, T., Sullivan, M. B., & Floge, S. A. (2022). Protist impacts on marine cyanovirocell metabolism. ISME Communications, 2(1). https://doi.org/10.1038/s43705-022-00169-6
Methods
Stehnach, M. R., Waisbord, N., Walkama, D. M., & Guasto, J. S. (2021). Viscophobic turning dictates microalgae transport in viscosity gradients. Nature Physics, 17(8), 926–930. https://doi.org/10.1038/s41567-021-01247-7

Henshaw, R. J. (2023) Chemotaxis of Vibrio alginolyticus to control/phage-infected Synechococcus exudates from 2020-2021 (VIC project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-12-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.885574.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation