| Data File(s) | Type | Description | Action |
|---|---|---|---|
885701_v1_multiplexed_chemotactic.csv (434.86 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 885701, version 1 | |
| Supplemental File(s) | Type | Description | Action |
Accumulations_PeakResponses.xls (8.50 KB) | Microsoft Excel | Maximum accumulation responses for each tested organism (V. alginolyticus, P. haloplanktis, E. coli) across a range of concentrations and chemicals. Corresponds to Fig. 4 in Stehnach et al. (Multiplexed microfluidic screening of bacterial chemotaxis). | |
Codes_MCD_PerformanceValidation.zip (20.47 KB) | ZIP Archive (ZIP) | MATLAB codes used to process and characterise the performance of the MCD channels. Includes a brief summary file. | |
MCD_AccumulationEvolution.xlsx (14.53 KB) | Microsoft Excel | Evolution of the cell positions of V. alginolyticus in a microfluidic channel with an evolving chemical gradient of a model chemoattractant (serine). Corresponds to Fig2d in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis) | |
MCD_Accumulations_Vibrio_Ecoli_Phalo_3bdfh.xlsx (88.20 KB) | Microsoft Excel | Accumulation plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). Corresponds to Figs 3bdfh in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis | |
MCD_CAD_Designs_AllLayers.dwg (323.40 KB) | ZIP Archive (ZIP) | Channel masks to reproduce the three wafer molds in order to fabricate the MCD (multiplexed chemotaxis device). | |
MCD_ChemicalConcentration_Gradient_Measurements.xlsx (453.72 KB) | Microsoft Excel | Measured chemical concentrations generated from the dilution microchannels for various driving pressures in each observation region | |
MCD_ChemicalEvolutionPressureRange.xlsx (36.25 MB) | Microsoft Excel | The uniformity of the chemostimulus gradient evolution across the various observation regions (C0-C5) was determined through fluorescent dye (fluorescein) visualization of stop-flow experiments with pressure 100 mbar (dilution layer) and 70 mbar (cell injection layer) | |
MCD_ChemotacticRepsonses_Vibrio_Ecoli_Phalo_ConditionalProbabilities.xls (1.13 MB) | Microsoft Excel | Conditional probability plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). C0 = 200uM, C1 = 49uM, C2 = 3.6uM, C3 = 0.12uM, C4 = 0.012uM, C5 = 0uM (Control) Corresponds to Figs 3aceg in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis | |
MCD_MicroMixerDegreeOfMixing.xlsx (10.55 KB) | Microsoft Excel | Mixing performance: The degree of mixing (DOM) was quantified for a long test channel having 29 herringbone cycles for two different flow rates: 22 uL\min and 222 uL\min using fluorescein dye | |
MCD_NoGradient_FixedGradient_Controls.xls (733.50 KB) | Microsoft Excel | Control chemotaxis experiments with V. alginolyticus to test reproducability between observation channels. Tested: no gradient (ASW for all inputs) and a fixed gradient of 200uM (serine) for all channels. Includes kymographs and accumulation curves, Fig. 9 and Fig. 2kl respectively of Stehnach et al (Multiplexed microfluidics of bacterial screening) | |
MCD_ObervationRegionSolutionDistribution.xlsx (11.30 KB) | Microsoft Excel | Chemostimulus distribution: The normalized measured widths of the chemical, cell, and buffer streams illustrate their high degree of symmetry across all observation channels | |
MCD_Valginolyticus_Chemotaxis_Serine_SingleAssay-MCD_Accumulation.xlsx (182.95 KB) | Microsoft Excel | Comparison of the chemotactic responses of V. alginolyticus between the single assays (SA) and the MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Accumulation index plots for both conditions (Fig f,h) Sorenson similarity measurement between the conditional probability plots of the two devices (Fig 2i) Comparison of the peak chemotactic response in each device (Fig 2j) Figure labels correspond to Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis) | |
MCD_Valginolyticus_Serine_MCDChemotaxisAssays_Fig2g.xlsx (249.54 KB) | Microsoft Excel | Conditional probability distributions of the chemotactic responses of V. alginolyticus in MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Corresponds to Fig 2g of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis) | |
MCD_Valginolyticus_Serine_SingleChemotaxisAssays_Fig2e.xlsx (1.34 MB) | Microsoft Excel | Conditional probability distributions of the chemotactic responses of V. alginolyticus in single assays (SA) for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Corresponds to Fig 2e of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis) |
Files
Filename: 885701_v1_multiplexed_chemotactic.csv (434.86 KB)
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 885701, version 1
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 885701, version 1
Supplemental Files
Filename: Accumulations_PeakResponses.xls (8.50 KB)
Type: Microsoft Excel
Description: Maximum accumulation responses for each tested organism (V. alginolyticus, P. haloplanktis, E. coli) across a range of concentrations and chemicals. Corresponds to Fig. 4 in Stehnach et al. (Multiplexed microfluidic screening of bacterial chemotaxis).
Type: Microsoft Excel
Description: Maximum accumulation responses for each tested organism (V. alginolyticus, P. haloplanktis, E. coli) across a range of concentrations and chemicals. Corresponds to Fig. 4 in Stehnach et al. (Multiplexed microfluidic screening of bacterial chemotaxis).
Filename: Codes_MCD_PerformanceValidation.zip (20.47 KB)
Type: ZIP Archive (ZIP)
Description: MATLAB codes used to process and characterise the performance of the MCD channels. Includes a brief summary file.
Type: ZIP Archive (ZIP)
Description: MATLAB codes used to process and characterise the performance of the MCD channels. Includes a brief summary file.
Filename: MCD_AccumulationEvolution.xlsx (14.53 KB)
Type: Microsoft Excel
Description: Evolution of the cell positions of V. alginolyticus in a microfluidic channel with an evolving chemical gradient of a model chemoattractant (serine). Corresponds to Fig2d in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Type: Microsoft Excel
Description: Evolution of the cell positions of V. alginolyticus in a microfluidic channel with an evolving chemical gradient of a model chemoattractant (serine). Corresponds to Fig2d in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Filename: MCD_Accumulations_Vibrio_Ecoli_Phalo_3bdfh.xlsx (88.20 KB)
Type: Microsoft Excel
Description: Accumulation plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). Corresponds to Figs 3bdfh in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis
Type: Microsoft Excel
Description: Accumulation plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). Corresponds to Figs 3bdfh in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis
Filename: MCD_CAD_Designs_AllLayers.dwg (323.40 KB)
Type: ZIP Archive (ZIP)
Description: Channel masks to reproduce the three wafer molds in order to fabricate the MCD (multiplexed chemotaxis device).
Type: ZIP Archive (ZIP)
Description: Channel masks to reproduce the three wafer molds in order to fabricate the MCD (multiplexed chemotaxis device).
Filename: MCD_ChemicalConcentration_Gradient_Measurements.xlsx (453.72 KB)
Type: Microsoft Excel
Description: Measured chemical concentrations generated from the dilution microchannels for various driving pressures in each observation region
Type: Microsoft Excel
Description: Measured chemical concentrations generated from the dilution microchannels for various driving pressures in each observation region
Filename: MCD_ChemicalEvolutionPressureRange.xlsx (36.25 MB)
Type: Microsoft Excel
Description: The uniformity of the chemostimulus gradient evolution across the various observation regions (C0-C5) was determined through fluorescent dye (fluorescein) visualization of stop-flow experiments with pressure 100 mbar (dilution layer) and 70 mbar (cell injection layer)
Type: Microsoft Excel
Description: The uniformity of the chemostimulus gradient evolution across the various observation regions (C0-C5) was determined through fluorescent dye (fluorescein) visualization of stop-flow experiments with pressure 100 mbar (dilution layer) and 70 mbar (cell injection layer)
Filename: MCD_ChemotacticRepsonses_Vibrio_Ecoli_Phalo_ConditionalProbabilities.xls (1.13 MB)
Type: Microsoft Excel
Description: Conditional probability plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). C0 = 200uM, C1 = 49uM, C2 = 3.6uM, C3 = 0.12uM, C4 = 0.012uM, C5 = 0uM (Control) Corresponds to Figs 3aceg in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis
Type: Microsoft Excel
Description: Conditional probability plots for chemotaxis experiments (MCD) for the following organism/chemical pairings: V. alginolyticus (VA) and phenol VA and leucine P. haloplanktis (PH) and serine E. coli (EC) and serine). C0 = 200uM, C1 = 49uM, C2 = 3.6uM, C3 = 0.12uM, C4 = 0.012uM, C5 = 0uM (Control) Corresponds to Figs 3aceg in Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis
Filename: MCD_MicroMixerDegreeOfMixing.xlsx (10.55 KB)
Type: Microsoft Excel
Description: Mixing performance: The degree of mixing (DOM) was quantified for a long test channel having 29 herringbone cycles for two different flow rates: 22 uL\min and 222 uL\min using fluorescein dye
Type: Microsoft Excel
Description: Mixing performance: The degree of mixing (DOM) was quantified for a long test channel having 29 herringbone cycles for two different flow rates: 22 uL\min and 222 uL\min using fluorescein dye
Filename: MCD_NoGradient_FixedGradient_Controls.xls (733.50 KB)
Type: Microsoft Excel
Description: Control chemotaxis experiments with V. alginolyticus to test reproducability between observation channels. Tested: no gradient (ASW for all inputs) and a fixed gradient of 200uM (serine) for all channels. Includes kymographs and accumulation curves, Fig. 9 and Fig. 2kl respectively of Stehnach et al (Multiplexed microfluidics of bacterial screening)
Type: Microsoft Excel
Description: Control chemotaxis experiments with V. alginolyticus to test reproducability between observation channels. Tested: no gradient (ASW for all inputs) and a fixed gradient of 200uM (serine) for all channels. Includes kymographs and accumulation curves, Fig. 9 and Fig. 2kl respectively of Stehnach et al (Multiplexed microfluidics of bacterial screening)
Filename: MCD_ObervationRegionSolutionDistribution.xlsx (11.30 KB)
Type: Microsoft Excel
Description: Chemostimulus distribution: The normalized measured widths of the chemical, cell, and buffer streams illustrate their high degree of symmetry across all observation channels
Type: Microsoft Excel
Description: Chemostimulus distribution: The normalized measured widths of the chemical, cell, and buffer streams illustrate their high degree of symmetry across all observation channels
Filename: MCD_Valginolyticus_Chemotaxis_Serine_SingleAssay-MCD_Accumulation.xlsx (182.95 KB)
Type: Microsoft Excel
Description: Comparison of the chemotactic responses of V. alginolyticus between the single assays (SA) and the MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Accumulation index plots for both conditions (Fig f,h) Sorenson similarity measurement between the conditional probability plots of the two devices (Fig 2i) Comparison of the peak chemotactic response in each device (Fig 2j) Figure labels correspond to Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Type: Microsoft Excel
Description: Comparison of the chemotactic responses of V. alginolyticus between the single assays (SA) and the MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Accumulation index plots for both conditions (Fig f,h) Sorenson similarity measurement between the conditional probability plots of the two devices (Fig 2i) Comparison of the peak chemotactic response in each device (Fig 2j) Figure labels correspond to Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Filename: MCD_Valginolyticus_Serine_MCDChemotaxisAssays_Fig2g.xlsx (249.54 KB)
Type: Microsoft Excel
Description: Conditional probability distributions of the chemotactic responses of V. alginolyticus in MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Corresponds to Fig 2g of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Type: Microsoft Excel
Description: Conditional probability distributions of the chemotactic responses of V. alginolyticus in MCD for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Includes: Corresponds to Fig 2g of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Filename: MCD_Valginolyticus_Serine_SingleChemotaxisAssays_Fig2e.xlsx (1.34 MB)
Type: Microsoft Excel
Description: Conditional probability distributions of the chemotactic responses of V. alginolyticus in single assays (SA) for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Corresponds to Fig 2e of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)
Type: Microsoft Excel
Description: Conditional probability distributions of the chemotactic responses of V. alginolyticus in single assays (SA) for the model chemoattract serine at 6 concentrations: 200, 49, 3.6, 0.12, 0.012,0uM. Corresponds to Fig 2e of Stehnach et al (Multiplexed microfluidic screening of bacterial chemotaxis)