©2020 Biological and Chemical Oceanography Data Management Office.A barcoded transposon mutant library (RB-TnSeq) of Ruegeria pomeroyi DSS-3 was screened on 23 substrates, each as a sole carbon source. In February of 2021 the RB-TnSeq library was grown in the laboratory in minimal medium with a substrate of interest, cultures were sequentially transfered four times to increase selection. Mutants with phenotypes that decreased fitness on the substrate of interest grow at a lower rate than mutants with no loss of fitness. Sequencing the barcodes of mutants after...
Show moreProcessing Notes from Researcher:
Sequence data were processed according to Wetmore et al (2015). Following quality control, an average of 35,090 unique barcodes mapped to insertions that fell within the interior 10 to 90% of R. pomeroyi coding sequences. In total, 55 million reads were mapped to insertions in 3,570 genes (out of 4,469 protein-encoding genes in the R. pomeroyi genome) with a median of 404,513 mapped reads per sample. Reads mapping to different insertion sites within the same coding sequence were pooled.
BCO-DMO Processing Notes:
Moran, M. A., Reisch, C. R., Mejia, C., Trujillo Rodriguez, L. (2023) Ruegeria pomeroyi RB-TnSeq Transponson Mutant Library Screen Experimental Data February 2021 (C-CoMP Marine Bacterial Transporters project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-05 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.893256.1 [access date]
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