©2020 Biological and Chemical Oceanography Data Management Office.High resolution growth screens were used to confirm the phenotype of Ruegeria pomeroyi DSS-3 transporter knockout mutants. Mutants which had demonstrated growth defects on a given substrate as sole carbon source during an initial growth screen were selected and used here. Each mutant was grown on the substrate(s) of interest along side a wildtype analog (pooled-TnSeq library). Growth curves were generated by reading the optical density at 600 nm hourly. The annotations of a transporter's cognate...
Show moreEach column heading provides the treatment condition and replicate. The first 4 letters are substrate abbreviation (provided below) followed by the bacterial identifier. The bacterial identity is either "WT" indicating the wildtype analog (pooled-RBTnSeq library) or four numbers indicating the locus tag of the disrupted gene (e.g. "1234" represents a mutant of "SPO1234"). Finally, the underscore and lowercase letter ("_a", "_b", "_c", "_d") indicates the biological replicate.
Values are optical density at 600 nm.
Moran, M. A., Reisch, C. R., Mejia, C., Trujillo Rodriguez, L. (2023) High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-04-18 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.894179.1 [access date]
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