The phyla Nitrospirota and Nitrospinota have received significant research attention due to their unique mitrogen metabolisms important to biogeochemical and industrial processes. These phyla are common inhabitants of marine and terrestrial subsurface environments and contain members capable of diverse physiologies in addition to nitrite oxidation and complete ammonia oxidation. We used phylogenomics and gene-based analysis with ancestral state reconstruction and gene-tree-species tree reconcili...
Show moreGenomic dataset collection, curation and quality control: This study used publicly available genome assemblies. Existing publicly available genome assemblies were downloaded from the National Center for Biotechnology Investigation (NCBI) and the Integrated Microbial Genomes (IMG) database of the U.S. Department of Energy’s Joint Genome Institute in June 2021. The Genome Taxonomy Database (GTDB) website (release 202) was used to access lists of NCBI assembly accession numbers for the following GTDB-assigned phyla: Nitrospinota, Nitrospinota_A (now called Tectomicrobia), Nitrospinota_B, Nitrospirota, Nitrospirota_A (Leptospirilla). The IMG assemblies were found using the same GTDB taxonomy classifier using the search function on the IMG website. IMG metagenome assemblies that were designated as “public” and “published” were also downloaded for these phyla. Duplicate entries between IMG and NCBI were manually removed.
Orcutt, B. N., D'Angelo, T. (2024) Collection of subsurface bacteria Nitrospirota and Nitrospinota genome data including IMG and NCBI accessions for sequence datasets in June 2021 (Slow Life in Crust project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/933610 [access date]
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