Dataset: Saanich Inlet field and laboratory methods related to environmental data acquired aboard the R/V John Strickland during two cruises in August, 2021 and June, 2022

Nutrients - Sample collection: Duplicate 15-mL polypropylene tubes were rinsed three times with 0.2-µm filtered seawater before collecting 10 mLs and freezing at -20 degrees C.

Nitrite - Sample collection:  Duplicate 125-mL HDPE bottles were rinsed three times with 0.2-µm filtered seawater before collecting 100 mLs and storing at room temperature in the dark.

Ammonium - Sample collection: Duplicate 50-mL glass tubes were rinsed three times with sample before collecting 40 mLs and adding 10 mLs orthophthaldialdehyde (OPA). Samples were stored in the dark prior to analysis.  

Oxygen - Sample collection:  Duplicate oxygen flasks were sampled from a dedicated Niskin bottle. Flasks were rinsed with sample while inverted then allowed to overfill three times their volume. Flasks immediately received 1-mL each manganous chloride reagent and alkaline sodium iodide flasks through a submerged spigot and were sealed with ground glass stoppers, using caution to avoid introduction of bubbles. After 30 seconds of vigorous shaking, flasks were stored in the dark with a deionized water seal around the stoppers. 

Nitrous Oxide - Sample collection:  Water was sampled through gas-tight Viton tubing into 20-mL glass vials filling from the bottom with three times overflow. Vials were closed bubble-free with deoxygenated butyl rubber stoppers, crimp-sealed and stored inverted in the dark at 4 °C until analysis.

Sulfide - Sample collection:  Water was sampled through gas-tight viton tubing into 12-mL glass exetainer vials, filling from the bottom with three times overflow and no headspace. Samples were immediately amended with N,N-dimethyl-p-phenylenediamine reagent and stored in the dark until analysis (within 6 hours) (following Cline, 1969).

DNA - Sample collection:  2.5 L of seawater were filtered onto 0.2-µm Sterivex filters. After addition of 1.2  mL of lysis buffer, filters were frozen on dry ice until the end of the day, when they were transferred to a -80 C freezer. 

RNA - Sample collection:  Between 2.25-2.5 L of seawater were filtered onto 0.2-µm Sterivex filters within 20 minutes of CTD recovery. Filters were flooded with RNA preservative and frozen on dry ice until the end of the day, when they were transferred to a   -80 C freezer.