Project: Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03)

Acronym/Short Name:MetZyme
Project Duration:2010-09 -2013-08
Geolocation:Tropical North Pacific along 150 degrees West from 18 degrees North to the equator

Description

MetZyme project researchers will determine the role of enzymatic activity in the cycling of trace metals. Specifically the research will address the following questions: (1) degradation of sinking particulate organic material in the Tropical North Pacific can be influenced by the ability of microbes to synthesize zinc proteases, which in turn is controlled by the abundance or availability of zinc, and (2) methylation of mercury is controlled, in part, by the activity of cobalt-containing enzymes, and therefore the supply of labile cobalt to the corrinoid-containing enzymes or co-factors responsible for methylation. To attain their goal, they will collect dissolved and particulate samples for trace metals and metalloenzymes from three stations along a biogeochemical gradient in the Tropical North Pacific (along 150 degrees West from 18 degrees North to the equator). Sinking particles from metal clean sediment traps will also be obtained. The samples will also be used to carry out shipboard incubation experiments using amendments of metals, metal-chelators, B12, and proteases to examine the sensitivity and metal limitation of heterotrophic, enzymatic degradation of organic matter within the oceanic "Twilight Zone" (100-500 m). This study will result in a novel metaproteomic/metalloenzyme datasets that should provide insights into the biogeochemical cycling of metals, as well as co-limitation of primary productivity and controls on the export of carbon from the photic zone. In addition to the final data being contributed to BCO-DMO, an online metaproteomic data server will be created so the community has access to the raw data files generated by this research.


DatasetLatest Version DateCurrent State
Total spectral count of proteins from R/V Kilo Moana cruise KM1128 for the METZYME expedition in the tropical North Pacific in 2011.2022-07-06Under revision
Concentrations of total dissolved trace metals (Fe, Mn, Zn, Ni, Cd) obtained using seaFAST preconcentration and ICP-MS from the R/V Kilo Moana cruise KM1128 in the Central Pacific Ocean in October 20112021-07-09Final no updates expected
Peptides and their spectral counts from KM1128 the METZYME expedition on R/V Kilo Moana in the tropical North Pacific in 20112021-03-30Under revision
Dissolved cobalt concentrations measured by cathodic stripping voltammetry from the MetZyme cruise KM1128 on R/V Kilo Moana in October 20112020-12-03Final no updates expected
Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 20162020-04-21Final no updates expected
Station locations for the global metaproteomic dataset from R/V Kilo Moana cruise KM1128, the METZYME expedition in the tropical North Pacific in 2011.2019-04-12Final no updates expected
Nutrients, targeted proteomics, and pigments from the METZYME cruise (KM1128)2018-05-24Final with updates expected
Hydrography data collected using the Trace Metal Rosette (TMR) on Metzyme cruise (R/V Kilo Moana KM1128) in the tropical North Pacific in October 20112017-10-11Preliminary and in progress
Processed hydrography data collected using the ship's CTD package during the Metzyme cruise (R/V Kilo Moana KM1128) in the tropical North Pacific in October 20112017-10-09Final no updates expected
FASTA file of protein identifications from R/V Kilo Moana KM1128 in the North Pacific during 2011 (MetZyme project)2017-07-14Final no updates expected
Rates of water column nitrification determined from bottle incubations with 15N tracers (15NH4Cl); samples collected on METZYME cruise.2016-05-27Final no updates expected
qPCR data for archaeal amoA ecotypes; samples collected on METZYME cruise.2016-05-24Final no updates expected

People

Principal Investigator: Carl Lamborg
Woods Hole Oceanographic Institution (WHOI)

Co-Principal Investigator: Mak A. Saito
Woods Hole Oceanographic Institution (WHOI)


Programs

U.S. GEOTRACES [U.S. GEOTRACES]